Resistance Phenotypes of Bacterial Strains Isolated from Patients Admitted to Surgical Wards
Oana-Maria Mişcă, Paula Bianca Maghiar, Liviu-Coriolan Mişcă, Bogdan Dan Totolici, Carmen Neamţu, Ionut Flaviu Faur, Liliana Dragomirescu, Daniel-Raul Chioibaş, Corina Dana Mişcă, Andreea-Adriana Neamţu, Aniela-Roxana Nodiţi, Cristina-Adriana Dehelean, Petrişor Zorin Crăiniceanu, Andrei Gheorghe Marius MotocOriginal article, no. 2, 2025
Article DOI: 10.21614/chirurgia.3053
Background/aim: This study aims to identify resistance phenotypes within the Plastic Surgery Department of a tertiary hospital in Romania. The goal is to guide the appropriate administration of antimicrobial therapy to optimize patient outcomes while minimizing the risk of multidrug-resistant bacterial strain development.
Methods: A prospective clinical study was conducted on 78 patients admitted to the Plastic Surgery Department. Pus samples were collected by attending physicians. Bacterial culture and identification using API cards were conducted. Antibiotic susceptibility testing was performed through the Kirby-Bauer disk diffusion method.
Results: A total of 100 bacterial strains were isolated from 78 clinical samples. The most frequently identified strains were: Staphylococcus aureus (32%), Klebsiella pneumoniae (21%), Escherichia coli (10%), Proteus mirabilis (10%), and Pseudomonas aeruginosa (10%). Among these, 20% of Escherichia coli and 28.5% of Klebsiella pneumoniae strains exhibited an extended-spectrum betalactamase (ESBL) phenotype. Resistance to fluoroquinolones was observed in 23.8% of Klebsiella pneumoniae and 10% of Escherichia coli strains. Aminoglycoside resistance was detected in 30% of Escherichia coli and 71.5% of Klebsiella pneumoniae strains. Analysis of Pseudomonas aeruginosa isolates revealed resistance to carbapenems â?" namely imipenem (40%), cephalosporins (50%), and fluoroquinolones (20%). Staphylococcus aureus strains were classified into different phenotypes, including methicillinresistant Staphylococcus aureus (62.5%), beta-lactamase producers (37.5%), aminoglycoside-resistant (78.1%), and fluoroquinolone-resistant strains (18.8%).
Conclusion: The findings highlight that the bacterial strains identified in this study demonstrated a high level of susceptibility to fluoroquinolones, suggesting their potential efficacy in antimicrobial therapy.
Methods: A prospective clinical study was conducted on 78 patients admitted to the Plastic Surgery Department. Pus samples were collected by attending physicians. Bacterial culture and identification using API cards were conducted. Antibiotic susceptibility testing was performed through the Kirby-Bauer disk diffusion method.
Results: A total of 100 bacterial strains were isolated from 78 clinical samples. The most frequently identified strains were: Staphylococcus aureus (32%), Klebsiella pneumoniae (21%), Escherichia coli (10%), Proteus mirabilis (10%), and Pseudomonas aeruginosa (10%). Among these, 20% of Escherichia coli and 28.5% of Klebsiella pneumoniae strains exhibited an extended-spectrum betalactamase (ESBL) phenotype. Resistance to fluoroquinolones was observed in 23.8% of Klebsiella pneumoniae and 10% of Escherichia coli strains. Aminoglycoside resistance was detected in 30% of Escherichia coli and 71.5% of Klebsiella pneumoniae strains. Analysis of Pseudomonas aeruginosa isolates revealed resistance to carbapenems â?" namely imipenem (40%), cephalosporins (50%), and fluoroquinolones (20%). Staphylococcus aureus strains were classified into different phenotypes, including methicillinresistant Staphylococcus aureus (62.5%), beta-lactamase producers (37.5%), aminoglycoside-resistant (78.1%), and fluoroquinolone-resistant strains (18.8%).
Conclusion: The findings highlight that the bacterial strains identified in this study demonstrated a high level of susceptibility to fluoroquinolones, suggesting their potential efficacy in antimicrobial therapy.
Keywords: bacteria, antibiotic resistance, antibiotic stewardship, surgical wound infection, fluoroquinolones, plastic surgery
