Impact of Allyl Disulfide on Oxidative Damage and Liver Regeneration in an Experimental Hepatectomy Model
M. Battal, A. Kartal, B. Citgez, B. Yilmaz, A. Akcakaya, O. KaratepeOriginal article, no. 2, 2015
Backgraund: We investigated the effects of allyl disulfide (agarlic extract) on tissue damage, regeneration, proliferationand oxidative damage in an experimental liver resectionmodel.Materials and Methods: In the study, 24 female Wistar albinorats weighing approximately 200-250 g were used. Group 1:The rats in the experimental group all received a 70%hepatectomy and were fed an Allyl disulfide (30 μg kg day,Allyl disulfide, Sigma-Aldrich, formula: C6H10S2, CASNumber: 2179-57-9, formula weight: 146.27 g mol) insupplement to a regular diet for 1 week both preoperativelyand postoperatively. Group 2: The rats in the control groupalso underwent a 70% hepatectomy and were given regularfood and water for 1 week both preop and postop. Group 3: Inthe sham group, all rats were sacrificed 7 days after surgery. Forbiochemical evaluation, SGOT, SGPT, bilirubin, CRP andMDA were studied. In a histopathological examination, thefattening of the liver tissue, existence of (macro-micro vesicular),fibrosis, pleomorphism at hepatocyte nuclei, portalinflammation, existence of intralobular inflammatory cells,dilation at sinusoids, congestion, congestion at the centralvein, regeneration, existence of Kupffer cells in the sinusoidallumen and ki-67 proliferation index at hepatocytes wereexamined. Results: A significant difference between group 1 and group2 was observed regarding the existence of regeneration,(p:0.06), the occurrence of nuclear pleomorphisms (p:0,001)and the fibroblast activity status (p:0.001). Significantdifferences were found between the experimental groups inregard to Kupffer cell increase and dilation and the hyperemiastatus in the sinusoid lumens (p:0.013 and p:0.001,respectively). In the Allyl disulfide group, the proliferationindex was significantly higher than that of the other groups(p:0,001), while the average plasma MDA value was lowerthan that of the other groups (p: 0,042). No significantdifferences were found among the groups with respect totissue MDA values (p:0,720). No significant difference wasfound for SGPT (ALT) and SGOT (AST) levels betweenGroup 1 and the other groups (p:0.247 and p:0.539, respectively).The average total bilirubin (T. Bili) values were 0,12,0,08 and 0,04 in the allyl disulfide group, control group andSham group, respectively. This difference among the groupsis statistically significant (p:0.001). The average directbilirubin (D. Bili) values were 0,06, 0,02 and 0,02 in theallyl disulfide group, control group and Sham group, respectively.This variation among the groups is also statisticallysignificant (0.001).Conclusion: We observed that the use of Allyl disulfidesupplementation after major hepatectomy has a positiveimpact on liver regeneration, proliferation and oxidativedamage.Abbreviations: Postop: post-operative, Preop: pre-operative,SGOT(AST): serum glutamic oxaloacetic transaminase,SGPT(ALT): serum glutamate-pyruvate transaminase, CRP:C- Reactive protein, MDA: Malondialdehyde, DAS: Garlicextract diallyl sulfide, AGE: aged garlic extract
