Effects of Acute Pancreatitis on Plasma Total and Lipid Bound Sialic Acid Levels: An Experimental Study in Rats
O.F. Ozkan, O. Cikman, E.C. Roach, A.S. Kemik, F. Gunes, M. KaraayvazOriginal article, no. 4, 2014
Background:We investigated the relationship between serumlevels of total sialic acid, lipid bound sialic acid and acutepancreatitis in a rat model of a common bile duct ligationinduced acute pancreatitis.Methods: Twenty five Sprague-Dawley male rats weighing 250-300g were divided into two groups (n=10: control, n=15:experimental). In the control group only a sham laparotomywas performed. In the experimental group, acute pancreatitiswas induced by common pancreatobiliary tract ligation. After36 hours the rats were killed and amylase, serum total sialicacid, lipid bound sialic acid and lipid profiles were measured.Histopathological confirmation of acute pancreatitis was doneusing hematoxylin and eosin staining.Results: Mean amylase, total sialic acid (TSA) and lipid boundsialic acid (LBSA) measurements in the experimental groupwere significantly higher than in the control group. There wasno significant difference in the lipid profiles between the twogroups.Conclusion: Increased levels of TSA and LBSA can be usefulas specific markers in the diagnosis of acute pancreatitisindependent of serum lipid profile.
